The Università del Piemonte Orientale “Amedeo Avogadro” – UPO, is a public Italian University established in 1998. It has 7 Departments and one School of Medicine, with approximately 12.000 students, 400 teachers/researchers, 350 members of technical and administrative staff, about 130 PhD students and 100 post-doc positions. The University offers a full range of academic disciplines thus embracing a large number of activities: basic and representative courses, laboratories, apprenticeships, training periods and international exchanges. High standard research and education represent the mission of the University. UPO is partner of several European research and education programme; it has been involved in more than 20 FP7 Projects (1 ERC grant) and 19 H2020 Projects (4 as Coordinator). In 2016 UPO has established the new Center of Excellence for Translational Research on Autoimmune and Allergic Diseases (CAAD) with new wide labs spaces, equipped with cutting edge facilities and where the scientists involved in FORCE REPAIR are affiliated and resident.
Role within Force Repair
The expertise of UPO is focused on immunology, both in vitro and in vivo. UPO will lead the studies of evaluation of immunological response in WP3 (lead partner) and WP4 by implementing next generation cytofluorimetric analysis and imaging mass cytometry (Hyperion). In WP3, UPO will be directly involved in defining the antibacterial properties and biofilm interaction of bio-ink and combined products. The antibacterial bio-ink and HA-DH will be studied against planktonic and biofilm models of sensitive and/or multi drug resistant (MRSA) strains. Furthermore, in WP3 PBMC from healthy donor will be co-cultured with bacteria (with/without the bio-ink) in order to evaluate the immunological response to the 3D-printed scaffold and its component. Proliferation and phenotype of cultured cells will be assessed by flow cytometry. In WP4 UPO will perform imaging mass cytometry analysis (Hyperion) on excised 3D printed scaffold embedded tissue and UPO will custom-design and validate specific panel for that purpose. The immune response provoked by the 3D printed dressing implanted subcutaneously will be evaluated on splenocytes by flow cytometry/real-time PCR.